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Strep-MBP-GRX samples were reduced with 10 mM DTT for 2 hours and the buffer was exchanged against 100 mM Tris/HCl p H 8.0, 1 mM EDTA using the PD MiniTrap G-25 desalting columns (GE Healthcare)

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That just reintroduces contaminants

Glutathione is a powerful antioxidant that supports detoxification, immune health, and protection against oxidative stress

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